In a vintage Strat or Tele type single coil the polepieces are magnets and will draw on the strings and pull them out of tune and have an adverse effect on sustain. The closer the neck and middle pickups are adjusted to the strings, the more they will adversely affect the sustain and pull the strings out of tune, especially the low E and A strings.
Because the string seems to be under more tension and feels stiffer near the bridge, it seems to me that the bridge pickup can be adjusted closer to the strings than the neck and middle pickups.
Alnico 5 polepieces seem to have a stronger magnetic field and adversely affect the sustain more than alnico 2 pickups which have a softer magnetic field.
Everyone who's owned both a Tele with two pickups and an Esquire with a single bridge pickup but no neck pickup has noticed that the Esquire sounds more lively, sustains better and plays a little more in tune. It's because the Esquire is missing the neck pickup so that missing pickup is not pulling on the strings.
I think humbuckers have much less of this adverse effect than single coils because the polepieces in a humbucker are not magnets and even though they do get "magnetized" by the actual magnet and do generate a magnetic field, that field must be weaker since they do not seem to pull the strings out of tune to nearly the degree that the magnets/polepieces in vintage style single coils do.
That's my story and I'm stickin' to it! :laugh2: