Tone difference between a 24.75 scale and a 24 scale

I agree scale length isn't the most important determinant of tone, but extra string tension certainly affects the sound as well as the feel.
That ¾" of difference between 24" and 24¾" is as big as the scale difference between 24¾" on a Les Paul and 25½" on a Strat or Tele.
IMO that's likely to be pretty noticeable when it come to tone.
 
In my opinion, no difference in tone really at all.

Harmonics will be different, but "tone" not much at all.
It will feel different, play a little bit different, notes on different places could ring differently perhaps... But it will not "sound" very much different at all.

That's my 2 cents worth. If there is any change, it will be like bumping the mid or treble on your amp EQ by a smidgeon. Less than a smidge ?
 
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teles and strats have different bridge pup angles with the tele being slightly more angled. extra treble on those old 50's amps isnt a bad thing sometimes
 
10s on a 25.5" will have higher tension than 10s on a 24.75" unless I'm missing your point.

My point is that everyone always attributes the difference in tone, and especially ease of play to scale length, but that is simply because a shorter scale carries less tension at a given gauge/tuning.

If you place 10's on an LP then you instantly have moved it in the direction of a 25.5 with 9's.
It's more about tension than it is about scale length.
 
tension is a thing for sure, but scale length is more than just tension. i use 11s on everything these days, other than a scalloped neck tele which has 10s. there is a difference in tension between the 25.5" guitars, 24.75" guitars, and my one 24" mustang. even mitigating that as much as one can, there is a definite difference in tone
 
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